Effect of Ampelopsis brevipedunculata (Maxim.) Trautv extract on skin barrier function

Seung-Jae Lee¹^,^*

¹Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56121, Korea.

^*Corresponding Author: Seung-Jae Lee, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56121, Korea, Republic of. E-mail: seung99@kribb.re.kr.

© Copyright 2023 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 26, 2022; Revised: Jan 26, 2023; Accepted: Jan 29, 2023

Published Online: Jan 30, 2023

Abstract

The skin is the largest organ that blocks invasion to the outside. Due to external stimuli, the skin barrier collapses and immune function abnormalities occur. This leads to skin diseases such as atopic dermatitis and psoriasis. Through this study, we attempted to develop a natural material that can be used for various dermatitis diseases. In this study, the gene expression of skin barrier-related proteins was confirmed using TNF-α/IFN-γ-stimulated HaCaT cells through real-time PCR. As a result, it was confirmed that the gene expression of skin barrier-related proteins and ceramide-related proteins was improved by the ABE-M. In this study, it was confirmed that the gene expression of FLG, IVL, and LOR, which are skin barrier-related proteins, was improved by ABE-M, and it was confirmed that the gene expression of the tight junction-related proteins, Occludin and ZO-1, was also increased. As such, it is expected that ABE-M can be used as a material for skin inflammation such as atopic dermatitis.

Keywords: skin barrier; tight junction; serine palmitoyltransferase; ceramidase; Ampelopsis brevipedunculata