Food and Life
Korean Society for Food Science of Animal Resources
Article

Safe bioremediation of chromium by a pseudomonas isolated from industrial waste water and detection of genes responsible for its degradation

Sara Ahmed, Gamal Hassan*, Tharwat Radwan
1Botany department, Faculty of Science, Fayoum University, Fayoum 63514, Egypt.
2Genetics department, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
3Botany department, Faculty of Science, Fayoum University, Fayoum 63514, Egypt.
*Corresponding Author: Gamal Mohamedin Hassan, E-mail: gmh01@fayoum.edu.eg.

© Copyright 2022 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 09, 2022; Revised: May 12, 2022; Accepted: May 23, 2022

Published Online: May 23, 2022

Abstract

Isolation of microorganisms capable of reducing toxic chromium (VI) into less toxic chromium (III)) has been given attention due to their significance in bioremediation of the contaminated sites. Five isolates out of the twelve isolates were selected according to their abilities to grow in the presence of high concentration of chromium. From the five bacterial isolates, isolates FAYP2 and FAYP3 were selected according to their higher reduction percent of toxic chromium (VI) into chromium (III) and their reduction percent was 60% and 70 % respectively. The isolates FAYP2 and FAYP3 were identified as P. aeruginosa and P. zhadogens, respectively, based on morphological and biochemical characterization and confirmed by molecular sequence analyses of amplified 16S rRNA genes with accession numbers OMO10239 and OMO019074. The results reveal that P. aeruginosa FAYP2 and P. zhadogens FAYP3 can be used for the bioremediation of wastewater effluent containing hexavalent chromium.

Keywords: Chromium; Pseudomonas spp; Bioremediation; Industrial waste water; 16SrRNA