Table 1. Operating conditions of gas chromatography for determination of fatty acid composition

Item	Condition
Instrument	Agilent 6890N, Agilent Technologies, USA
Column	Omegawax250(30 mx 0.25 mm id, 0.25 μm film thickness; Supelco, Bellefonte, PA, USA)
Detector	Flame Ionization Detector
Carrier gas	Helium (99.99%, Research purity)
Column flow rate	1.2 mL/min
Split ratio	100:1, 1 μL (Injection volume)
Injection port temperature	250°C
Detection port temperature	260°C
Oven temperature	150°C, hold for 2min4°C/min up to 220°C, hold for 30 min